hi i need help and currently pursuing my bsc I need help in a question.
Question
Determine the % ionisation of ibuprofen in blood(ph 7.4).
The pka of thiol group in of an active site cysteine residue if determined to be 8.5. Determine the ratio of
protonated(thiol) to deprotonated (thiolate) groups at pH 7.
Would sincerely appreciate your help.
Regards
Shanti
Hi Shanti
use Henderson–Hasselbalch equation
pH =pKa - log[deprotonated thiol ]/[protonated thiol]